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Immunogenetics. Dynamic profiling of the protein life cycle in response to pathogens.
Jovanovic, Marko; Rooney, Michael S; Mertins, Philipp; Przybylski, Dariusz; Chevrier, Nicolas; Satija, Rahul; Rodriguez, Edwin H; Fields, Alexander P; Schwartz, Schraga; Raychowdhury, Raktima; Mumbach, Maxwell R; Eisenhaure, Thomas; Rabani, Michal; Gennert, Dave; Lu, Diana; Delorey, Toni; Weissman, Jonathan S; Carr, Steven A; Hacohen, Nir; Regev, Aviv.
Afiliación
  • Jovanovic M; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Rooney MS; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Mertins P; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Przybylski D; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Chevrier N; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Harvard Faculty of Arts and Sciences Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA.
  • Satija R; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Rodriguez EH; Department of Cellular and Molecular Pharmacology, California Institute for Quantitative Biomedical Research, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Fields AP; Department of Cellular and Molecular Pharmacology, California Institute for Quantitative Biomedical Research, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Schwartz S; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Raychowdhury R; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Mumbach MR; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Eisenhaure T; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, MA 02114, USA.
  • Rabani M; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Gennert D; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Lu D; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Delorey T; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Weissman JS; Department of Cellular and Molecular Pharmacology, California Institute for Quantitative Biomedical Research, University of California, San Francisco, San Francisco, CA 94158, USA. Howard Hughes Medical Institute (HHMI), University of California, San Francisco, San Francisco, CA 94158, USA.
  • Carr SA; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Hacohen N; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, MA 02114, USA. Harvard Medical School, Boston, MA 02115, USA. aregev@broad.mit.edu nhacohen@mgh.harvard.edu.
  • Regev A; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02140, USA. HHMI, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02140, USA. aregev@broad.mit.edu nhacohen@mgh.harvard.edu.
Science ; 347(6226): 1259038, 2015 Mar 06.
Article en En | MEDLINE | ID: mdl-25745177
ABSTRACT
Protein expression is regulated by the production and degradation of messenger RNAs (mRNAs) and proteins, but their specific relationships remain unknown. We combine measurements of protein production and degradation and mRNA dynamics so as to build a quantitative genomic model of the differential regulation of gene expression in lipopolysaccharide-stimulated mouse dendritic cells. Changes in mRNA abundance play a dominant role in determining most dynamic fold changes in protein levels. Conversely, the preexisting proteome of proteins performing basic cellular functions is remodeled primarily through changes in protein production or degradation, accounting for more than half of the absolute change in protein molecules in the cell. Thus, the proteome is regulated by transcriptional induction for newly activated cellular functions and by protein life-cycle changes for remodeling of preexisting functions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biosíntesis de Proteínas / Células Dendríticas / Células de la Médula Ósea / Interacciones Huésped-Patógeno / Simulación de Dinámica Molecular / Proteolisis Límite: Animals Idioma: En Revista: Science Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biosíntesis de Proteínas / Células Dendríticas / Células de la Médula Ósea / Interacciones Huésped-Patógeno / Simulación de Dinámica Molecular / Proteolisis Límite: Animals Idioma: En Revista: Science Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos