Real-time fluorescence assays of alkaline phosphatase and ATP sulfurylase activities based on a novel PPi fluorescent probe.

Wang, Xiaobo; Zhang, Zhiyang; Ma, Xiaoyan; Wen, Jinghan; Geng, Zhirong; Wang, Zhilin

Wang, Xiaobo; Zhang, Zhiyang; Ma, Xiaoyan; Wen, Jinghan; Geng, Zhirong; Wang, Zhilin.

Afiliación

Wang X; State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China.
Zhang Z; State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China.
Ma X; State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China.
Wen J; State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China.
Geng Z; State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China. Electronic address: gengzr@nju.edu.cn.
Wang Z; State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China. Electronic address: wangzl@nju.edu.cn.

Talanta ; 137: 156-60, 2015 May.

Article en En | MEDLINE | ID: mdl-25770619

Asunto(s)

Fosfatasa Alcalina/metabolismo; Difosfatos/química; Difosfatos/metabolismo; Pruebas de Enzimas/métodos; Colorantes Fluorescentes/química; Sulfato Adenililtransferasa/metabolismo; Fosfatasa Alcalina/antagonistas & inhibidores; Fosfatasa Alcalina/química; Antracenos/química; Inhibidores Enzimáticos/farmacología; Espectrometría de Fluorescencia; Sulfato Adenililtransferasa/antagonistas & inhibidores; Sulfato Adenililtransferasa/química; Factores de Tiempo; Zinc/análisis; Zinc/química

Palabras clave

ATP sulfurylase; Alkaline phosphatase; Fluorescent probe; PPi; Real-time fluorescence assay

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sulfato Adenililtransferasa / Difosfatos / Fosfatasa Alcalina / Pruebas de Enzimas / Colorantes Fluorescentes Idioma: En Revista: Talanta Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos

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