Structural insights into the role of rRNA modifications in protein synthesis and ribosome assembly.

Polikanov, Yury S; Melnikov, Sergey V; Söll, Dieter; Steitz, Thomas A

Polikanov, Yury S; Melnikov, Sergey V; Söll, Dieter; Steitz, Thomas A.

Afiliación

Polikanov YS; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA.
Melnikov SV; Howard Hughes Medical Institute at Yale University, New Haven, CT, USA.
Söll D; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA.
Steitz TA; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA.

Nat Struct Mol Biol ; 22(4): 342-344, 2015 Apr.

Article en En | MEDLINE | ID: mdl-25775268

ABSTRACT

ABSTRACT

We report crystal structures of the Thermus thermophilus ribosome at 2.3- to 2.5-Å resolution, which have enabled modeling of rRNA modifications. The structures reveal contacts of modified nucleotides with mRNA and tRNAs or protein pY, and contacts within the ribosome interior stabilizing the functional fold of rRNA. Our work provides a resource to explore the roles of rRNA modifications and yields a more comprehensive atomic model of a bacterial ribosome.

Asunto(s)

ARN Bacteriano/química; ARN Ribosómico/química; Ribosomas/química; Thermus thermophilus/genética; Cristalografía por Rayos X; Modelos Moleculares; Biosíntesis de Proteínas; Estructura Terciaria de Proteína; ARN Mensajero/química; ARN de Transferencia/química

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribosomas / ARN Bacteriano / ARN Ribosómico / Thermus thermophilus Idioma: En Revista: Nat Struct Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos

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