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An accurate and sensitive Coomassie Brilliant Blue G-250-based assay for protein determination.
Grintzalis, Konstantinos; Georgiou, Christos D; Schneider, Yves-Jacques.
Afiliación
  • Grintzalis K; Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences, Université Catholique de Louvain (UCL), Louvain-la-Neuve, Belgium. Electronic address: kgrintzalis@gmail.com.
  • Georgiou CD; Department of Biology, Section of Genetics, Cell Biology and Development, University of Patras, Patras, Greece.
  • Schneider YJ; Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences, Université Catholique de Louvain (UCL), Louvain-la-Neuve, Belgium.
Anal Biochem ; 480: 28-30, 2015 Jul 01.
Article en En | MEDLINE | ID: mdl-25837770
ABSTRACT
In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 µg protein.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colorantes de Rosanilina / Proteínas Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Biochem Año: 2015 Tipo del documento: Article
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colorantes de Rosanilina / Proteínas Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Biochem Año: 2015 Tipo del documento: Article