[Auto-inducible expression system based on the SigB-dependent ohrB promoter in Bacillus subtilis].
Mol Biol (Mosk)
; 48(6): 970-6, 2014.
Article
en Ru
| MEDLINE
| ID: mdl-25845237
ABSTRACT
The reliable production of heterologous proteins is important in the field of industrial biotechnology. This can be achieved by applying auto-inducible gene expression systems. The development of a Bacillus subtilis expression plasmid harboring SigB-dependent ohrB promoter was reported. The expression system was subjected to high cell density cultivation to produce xylanase as a stable model protein. The recombinant strain was cultured in a synthetic medium containing glucose as the carbon source. The exponential fed-batch feeding strategy was applied to prevent substrate inhibition. A sharp increase of xylanase activity (about 6-fold) at the end of the fermentation was observed as a result of sigma factor B (SigB) protein activation, supporting auto-inducibility of the expression system. For the control strain a specific induction of the xylanase activity was not observed. The recombinant strain was capable to offer a 5-fold increase in xylanase activity in comparison with the control strain. In addition, the constructed system displayed catabolite repression resistance ability. This SigB-dependent expression system can be considered as a biotechnology tool and an alternative to eliminate the cost of conventional inducers, e.g. isopropyl-ß-galactopyranoside.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plásmidos
/
Factor sigma
/
Bacillus subtilis
/
Proteínas Bacterianas
/
Regulación Bacteriana de la Expresión Génica
/
Regiones Promotoras Genéticas
/
Endo-1,4-beta Xilanasas
Tipo de estudio:
Prognostic_studies
Idioma:
Ru
Revista:
Mol Biol (Mosk)
Año:
2014
Tipo del documento:
Article