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In vivo targeting of de novo DNA methylation by histone modifications in yeast and mouse.
Morselli, Marco; Pastor, William A; Montanini, Barbara; Nee, Kevin; Ferrari, Roberto; Fu, Kai; Bonora, Giancarlo; Rubbi, Liudmilla; Clark, Amander T; Ottonello, Simone; Jacobsen, Steven E; Pellegrini, Matteo.
Afiliación
  • Morselli M; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
  • Pastor WA; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
  • Montanini B; Biochemistry and Molecular Biology Unit, Department of Life Sciences, Laboratory of Functional Genomics and Protein Engineering, Parma, Italy.
  • Nee K; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
  • Ferrari R; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
  • Fu K; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
  • Bonora G; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
  • Rubbi L; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, United States.
  • Clark AT; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
  • Ottonello S; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
  • Jacobsen SE; Biochemistry and Molecular Biology Unit, Department of Life Sciences, Laboratory of Functional Genomics and Protein Engineering, Parma, Italy.
  • Pellegrini M; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
Elife ; 4: e06205, 2015 Apr 07.
Article en En | MEDLINE | ID: mdl-25848745
ABSTRACT
Methylation of cytosines (5(me)C) is a widespread heritable DNA modification. During mammalian development, two global demethylation events are followed by waves of de novo DNA methylation. In vivo mechanisms of DNA methylation establishment are largely uncharacterized. Here, we use Saccharomyces cerevisiae as a system lacking DNA methylation to define the chromatin features influencing the activity of the murine DNMT3B. Our data demonstrate that DNMT3B and H3K4 methylation are mutually exclusive and that DNMT3B is co-localized with H3K36 methylated regions. In support of this observation, DNA methylation analysis in yeast strains without Set1 and Set2 shows an increase of relative 5(me)C levels at the transcription start site and a decrease in the gene-body, respectively. We extend our observation to the murine male germline, where H3K4me3 is strongly anti-correlated while H3K36me3 correlates with accelerated DNA methylation. These results show the importance of H3K36 methylation for gene-body DNA methylation in vivo.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Cromatina / Histonas / Metilación de ADN / ADN (Citosina-5-)-Metiltransferasas Límite: Animals Idioma: En Revista: Elife Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Cromatina / Histonas / Metilación de ADN / ADN (Citosina-5-)-Metiltransferasas Límite: Animals Idioma: En Revista: Elife Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos