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The c-cbl proto-oncogene is preferentially expressed in thymus and testis tissue and encodes a nuclear protein.
Langdon, W Y; Hyland, C D; Grumont, R J; Morse, H C.
Afiliación
  • Langdon WY; Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, South Australia.
J Virol ; 63(12): 5420-4, 1989 Dec.
Article en En | MEDLINE | ID: mdl-2585608
ABSTRACT
Cas NS-1 is an acutely transforming murine retrovirus that induces early B-lineage lymphomas and occasional myeloid leukemias. The transforming sequence of this virus, v-cbl, shows no homology to known oncogenes but has some similarities to the yeast transcriptional factor GCN4. In this study we used a v-cbl probe to analyze mRNAs from a wide range of murine and human hemopoietic tumor cell lines and detected an 11-kilobase mRNA in all lineages. In normal mouse tissues the expression of c-cbl was highest in testis and thymus tissues, the predominant species in testis tissue being a 3.5-kilobase mRNA. The v-cbl oncogene was inserted into a bacterial expression vector to produce protein for the immunization of rabbits. Affinity-purified v-cbl antibodies identified abundant levels of p100gag-cbl in Cas NS-1-transformed fibroblasts and lower levels of a 135-kilodalton protein (p135c-cbl) in both normal and transformed cells. Subcellular fractionation showed that p100gag-cbl and p135c-cbl are both located in the nucleus and retained following 420 mM salt extraction. These results indicate that the translational product of a c-cbl is a 135-kilodalton nuclear protein.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Testículo / Timo / Transcripción Genética / Proto-Oncogenes / Proteínas Nucleares / Expresión Génica / Proteínas Oncogénicas de Retroviridae / Transformación Celular Neoplásica Tipo de estudio: Prognostic_studies Límite: Animals / Humans / Male Idioma: En Revista: J Virol Año: 1989 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Testículo / Timo / Transcripción Genética / Proto-Oncogenes / Proteínas Nucleares / Expresión Génica / Proteínas Oncogénicas de Retroviridae / Transformación Celular Neoplásica Tipo de estudio: Prognostic_studies Límite: Animals / Humans / Male Idioma: En Revista: J Virol Año: 1989 Tipo del documento: Article