Role for RNA:DNA hybrids in origin-independent replication priming in a eukaryotic system.
Proc Natl Acad Sci U S A
; 112(18): 5779-84, 2015 May 05.
Article
en En
| MEDLINE
| ID: mdl-25902524
ABSTRACT
DNA replication initiates at defined replication origins along eukaryotic chromosomes, ensuring complete genome duplication within a single S-phase. A key feature of replication origins is their ability to control the onset of DNA synthesis mediated by DNA polymerase-α and its intrinsic RNA primase activity. Here, we describe a novel origin-independent replication process that is mediated by transcription. RNA polymerase I transcription constraints lead to persistent RNADNA hybrids (R-loops) that prime replication in the ribosomal DNA locus. Our results suggest that eukaryotic genomes have developed tools to prevent R-loop-mediated replication events that potentially contribute to copy number variation, particularly relevant to carcinogenesis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
ADN Ribosómico
/
ARN
/
Ribonucleasa H
/
Inestabilidad Genómica
/
Replicación del ADN
Límite:
Humans
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2015
Tipo del documento:
Article
País de afiliación:
España