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The influence of poly(ethylene glycol) ether tetrasuccinimidyl glutarate on the structural, physical, and biological properties of collagen fibers.
Sanami, Mohammad; Sweeney, India; Shtein, Zvi; Meirovich, Sigal; Sorushanova, Anna; Mullen, Anne Maria; Miraftab, Mohsen; Shoseyov, Oded; O'Dowd, Colm; Pandit, Abhay; Zeugolis, Dimitrios I.
Afiliación
  • Sanami M; Vornia Biomaterials Ltd., Galway, Ireland.
  • Sweeney I; Institute of Materials Research and Innovation, University of Bolton, Bolton, UK.
  • Shtein Z; Vornia Biomaterials Ltd., Galway, Ireland.
  • Meirovich S; Institute of Materials Research and Innovation, University of Bolton, Bolton, UK.
  • Sorushanova A; Vornia Biomaterials Ltd., Galway, Ireland.
  • Mullen AM; Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Miraftab M; Vornia Biomaterials Ltd., Galway, Ireland.
  • Shoseyov O; Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • O'Dowd C; Regenerative, Modular and Developmental Engineering Laboratory (REMODEL), Biosciences Research Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.
  • Pandit A; Network of Excellence for Functional Biomaterials (NFB), Biosciences Research Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.
  • Zeugolis DI; CURAM-Centre for Research in Medical Devices, Biosciences Research Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.
J Biomed Mater Res B Appl Biomater ; 104(5): 914-22, 2016 07.
Article en En | MEDLINE | ID: mdl-25952265
Various chemical, natural, or synthetic in origin, crosslinking methods have been proposed over the years to stabilise collagen fibers. However, an optimal method has yet to be identified. Herein, we ventured to assess the potential of 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate, as opposed to glutaraldehyde (GTA), genipin and carbodiimide, on the structural, physical and biological properties of collagen fibers. The 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate induced an intermedium surface smoothness, denaturation temperature and swelling. The 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate fibers had significantly higher stress at break values than the carbodiimide fibers, but significantly lower than the GTA and genipin fibers. With respect to strain at break, no significant difference was observed among the crosslinking treatments. The 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate fibers exhibited significantly higher cell metabolic activity and DNA concentration that all other crosslinking treatments, promoted consistently cellular elongation along the longitudinal fiber axis and by day 7 they were completely covered by cells. Collectively, this work clearly demonstrates the potential of 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate as collagen crosslinker. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 914-922, 2016.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polietilenglicoles / Ensayo de Materiales / Colágeno / Reactivos de Enlaces Cruzados / Fibroblastos / Glutaratos Límite: Humans Idioma: En Revista: J Biomed Mater Res B Appl Biomater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2016 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polietilenglicoles / Ensayo de Materiales / Colágeno / Reactivos de Enlaces Cruzados / Fibroblastos / Glutaratos Límite: Humans Idioma: En Revista: J Biomed Mater Res B Appl Biomater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2016 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Estados Unidos