[Influence of 20 % sulfur hexafluoride (SF6) on human corneal endothelial cells : An in vitro study]. / Einfluss von 20 % Schwefelhexafluorid (SF6) auf humane korneale Endothelzellen : Eine In-vitro-Studie.
Ophthalmologe
; 113(1): 52-7, 2016 Jan.
Article
en De
| MEDLINE
| ID: mdl-26025298
ABSTRACT
BACKGROUND:
In posterior lamellar keratoplasties, such as Descemet membrane endothelial keratoplasty (DMEK) and Descemet's stripping automated endothelial keratoplasty (DSAEK) an air bubble is left inside the anterior chamber to promote graft attachment during the early postoperative period. In the case of insufficient graft adhesion a renewed intracameral air injection is often necessary. The use of sulfur hexafluoride diluted with air (SF6 20 %) as an alternative to pure air may further enhance graft attachment and reduce the rebubbling rate. The effect of SF6 20 % on corneal endothelium is currently unclear and was therefore examined in vitro. MATERIAL ANDMETHODS:
For this study 12 human corneoscleral discs were mounted in artificial anterior chambers, the systems were continuously filled with culture medium and the anterior chambers with air (n = 5) or SF6 20 % (n = 7) as tamponade. After 6 days of storage in the incubator endothelial cell density, toxicity on endothelial cells and corneal thickness were evaluated.RESULTS:
There were no significant differences in endothelial cell loss (p = 1.000), endothelial cell count (p = 0.648), toxicity on endothelial cells (p = 0.048) and central corneal thickness (p = 0.905) between the two groups after 1 week. The level of significance was defined as p ≤ 0.05 and adjusted to p ≤ 0.0056 according to the Bonferroni correction for multiple testing.CONCLUSION:
The use of SF6 20 % as tamponade in the anterior chamber for posterior lamellar keratoplasty can be proposed as a safe alternative to pure air filling related to endothelial cell loss. Increased toxic effects on the corneal endothelium by SF6 20 % were not detected in this study; however, further prospective clinical trials are needed to examine the long-term effects in humans.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hexafluoruro de Azufre
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Endotelio Corneal
/
Células Endoteliales
Límite:
Aged
/
Female
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Humans
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Male
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Middle aged
Idioma:
De
Revista:
Ophthalmologe
Asunto de la revista:
OFTALMOLOGIA
Año:
2016
Tipo del documento:
Article