A Modular Synthesis of Modified Phosphoanhydrides.
Chemistry
; 21(28): 10116-22, 2015 Jul 06.
Article
en En
| MEDLINE
| ID: mdl-26033174
ABSTRACT
Phosphoanhydrides (P-anhydrides) are ubiquitously occurring modifications in nature. Nucleotides and their conjugates, for example, are among the most important building blocks and signaling molecules in cell biology. To study and manipulate their biological functions, a diverse range of analogues have been developed. Phosphate-modified analogues have been successfully applied to study proteins that depend on these abundant cellular building blocks, but very often both the preparation and purification of these molecules are challenging. This study discloses a general access to P-anhydrides, including different nucleotide probes, that greatly facilitates their preparation and isolation. The convenient and scalable synthesis of, for example, (18) O labeled nucleoside triphosphates holds promise for future applications in phosphoproteomics.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfatos
/
Anhídridos
/
Nucleósidos
/
Nucleótidos
Idioma:
En
Revista:
Chemistry
Asunto de la revista:
QUIMICA
Año:
2015
Tipo del documento:
Article