Glucuronic acid in Arabidopsis thaliana xylans carries a novel pentose substituent.
Int J Biol Macromol
; 79: 807-12, 2015 Aug.
Article
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| MEDLINE
| ID: mdl-26047894
ABSTRACT
Glucuronic acids in Arabidopsis thaliana xylans exist in 4-O-methylated (MeGlcA) and non-methylated (GlcA) forms at a ratio of about 32. The matrix-assisted laser desorption/ionization mass spectrometry analysis of the endoxylanase liberated acidic oligosaccharides from the Arabidopsis inflorescence stem showed that two peaks with GlcA (GlcA-Xyl4Ac1 and GlcA-Xyl5Ac2) had abnormally high intensities, as well as different tandem mass spectra, than their 4-O-methylated counterparts. These peaks were interestingly enriched in the xylan biosynthesis mutant irx7 and irx9-1. Multi-stages fragmentation analysis using negative ion electrospray-ion trap mass spectrometry indicated that this GlcA was further carrying a pentose residue in the glucuronoxylan-derived oligosaccharide from irx9-1. The structure was also identified in Arabidopsis wild type. The results prove evidence of a new pentose substitution on the GlcA residue of Arabidopsis GX, which is likely present in the primary walls.
Palabras clave
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pentosas
/
Pentosiltransferasa
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Xilanos
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Arabidopsis
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Ácido Glucurónico
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Proteínas de Arabidopsis
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2015
Tipo del documento:
Article