High dose of TNF-α suppressed osteogenic differentiation of human dental pulp stem cells by activating the Wnt/ß-catenin signaling.
J Mol Histol
; 46(4-5): 409-20, 2015 Oct.
Article
en En
| MEDLINE
| ID: mdl-26115593
ABSTRACT
Dental pulp stem cells (DPSCs) were a clonogenic, highly proliferative cells capable of self-renewal and multi-lineage differentiation including chondrocytes, adipocytes, neural cells and osteoblasts, which make it an attractive choice for bone regeneration and repair of craniofacial defects. Recent studies showed that tumor necrosis factor α (TNF-α) may affect osteoclastogenesis and bone formation. However, the effect and mechanism of TNF-α on DPSCs is not clear. In this study, we found that low dose TNF-α promoted mineralization and high dose TNF-α suppressed osteogenic differentiation of DPSCs. Levels of ALP, Osteopontin, Osteocalcin, Osterix and Runx2 were up-regulated in DPSCs treated with TNF-α at low concentration, while down-regulated in DPSCs treated with TNF-α at high concentration. Blockade of Wnt/ß-catenin signaling reversed the inhibitory effect observed on osteogenic differentiation of DPSCs treated with TNF-α at high concentration. In addition, we did not detect any proliferative effect of TNF-α on DPSCs by cell cycle and cell counts analysis. In summary, our data suggested that high concentration TNF-α suppressed mineralization and mineralization-related gene expressions through the Wnt/ß-catenin signaling in DPSCs. Our findings may provide a foundation for autologous transplantation of DPSCs.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteogénesis
/
Células Madre
/
Diferenciación Celular
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Factor de Necrosis Tumoral alfa
/
Pulpa Dental
/
Vía de Señalización Wnt
Límite:
Adolescent
/
Adult
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Humans
Idioma:
En
Revista:
J Mol Histol
Asunto de la revista:
HISTOCITOQUIMICA
Año:
2015
Tipo del documento:
Article