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Measurement of catecholamines in rat and mini-pig plasma and urine by liquid chromatography-tandem mass spectrometry coupled with solid phase extraction.
He, Huaibing; Carballo-Jane, Ester; Tong, Xinchun; Cohen, Lucinda H.
Afiliación
  • He H; Discovery Bioanalytics Group, Department of Pharmacokinetics, Pharmacodynamics and Drug Metabolism, USA.
  • Carballo-Jane E; Department of In Vivo Pharmacology Merck Research Laboratories Rahway, NJ 07065, USA.
  • Tong X; Discovery Bioanalytics Group, Department of Pharmacokinetics, Pharmacodynamics and Drug Metabolism, USA.
  • Cohen LH; Discovery Bioanalytics Group, Department of Pharmacokinetics, Pharmacodynamics and Drug Metabolism, USA. Electronic address: lucinda_cohen@merck.com.
Article en En | MEDLINE | ID: mdl-26117309
ABSTRACT
A tandem mass spectrometry method combined with an ion-pair chromatographic separation after weak cation exchange solid phase sample extraction for epinephrine (E), norepinephrine (NE) and dopamine (DA) has been developed. Two surrogate matrixes for plasma and urine as well as stable isotope labeled internal standards were utilized for quantitation. The observed dynamic range of E, NE and DA was 0.025-100ng/ml for plasma, and 0.25-1000ng/ml for urine with a r(2) regression coefficient >0.99. Extraction recoveries were greater than 60% and the lower limit of quantitation was 25pg/ml for all three analytes in plasma. This method provided excellent sensitivity and selectivity for use with small sample volumes (≤25uL), enabling high-throughput pharmacodynamic animal model development and screening of adverse effects.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Catecolaminas / Cromatografía Líquida de Alta Presión / Espectrometría de Masas en Tándem / Extracción en Fase Sólida Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Catecolaminas / Cromatografía Líquida de Alta Presión / Espectrometría de Masas en Tándem / Extracción en Fase Sólida Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos