Automated Identification and Localization of Hematopoietic Stem Cells in 3D Intravital Microscopy Data.
Stem Cell Reports
; 5(1): 139-53, 2015 Jul 14.
Article
en En
| MEDLINE
| ID: mdl-26120058
ABSTRACT
Measuring three-dimensional (3D) localization of hematopoietic stem cells (HSCs) within the bone marrow microenvironment using intravital microscopy is a rapidly expanding research theme. This approach holds the key to understanding the detail of HSC-niche interactions, which are critical for appropriate stem cell function. Due to the complex tissue architecture of the bone marrow and to the progressive introduction of scattering and signal loss at increasing imaging depths, there is no ready-made software to handle efficient segmentation and unbiased analysis of the data. To address this, we developed an automated image analysis tool that simplifies and standardizes the biological interpretation of 3D HSC microenvironment images. The algorithm identifies HSCs and measures their localization relative to surrounding osteoblast cells and bone collagen. We demonstrate here the effectiveness, consistency, and accuracy of the proposed approach compared to current manual analysis and its wider applicability to analyze other 3D bone marrow components.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Médula Ósea
/
Células Madre Hematopoyéticas
/
Nicho de Células Madre
/
Microscopía Intravital
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Stem Cell Reports
Año:
2015
Tipo del documento:
Article