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Introduction of Zinc-salt Fixation for Effective Detection of Immune Cell-related Markers by Immunohistochemistry.
Mori, Hidetoshi; Soonsawad, Pan; Schuetter, Louis; Chen, Qian; Hubbard, Neil E; Cardiff, Robert D; Borowsky, Alexander D.
Afiliación
  • Mori H; Center of Comparative Medicine, University of California, Davis, California, USA.
  • Soonsawad P; Center of Comparative Medicine, University of California, Davis, California, USA.
  • Schuetter L; Center of Comparative Medicine, University of California, Davis, California, USA.
  • Chen Q; Center of Comparative Medicine, University of California, Davis, California, USA.
  • Hubbard NE; Center of Comparative Medicine, University of California, Davis, California, USA.
  • Cardiff RD; Center of Comparative Medicine, University of California, Davis, California, USA Department of Pathology and Laboratory Medicine, School of Medicine, University of California, Davis, California, USA.
  • Borowsky AD; Center of Comparative Medicine, University of California, Davis, California, USA Department of Pathology and Laboratory Medicine, School of Medicine, University of California, Davis, California, USA adborowsky@ucdavis.edu hmori@ucdavis.edu.
Toxicol Pathol ; 43(6): 883-9, 2015 Aug.
Article en En | MEDLINE | ID: mdl-26157038
ABSTRACT
Tissue localization of immune cells is critical to the study of disease processes in mouse models of human diseases. However, immunohistochemistry (IHC) for immune cell phenotyping in mouse tissue sections presents specific technical challenges. For example, CD4 and CD8 have been difficult to detect using IHC on formalin-fixed and paraffin-embedded mouse tissue, prompting alternative methods. We investigated the use of formalin-free zinc-salt fixation (ZN) and optimized IHC protocols for detecting a panel of immune cell-related markers (CD3, CD4, CD8, Foxp3, B220, F4/80, CD68, and major histocompatibility complex [MHC] class-I, MHC class-II, and Gr-1). The IHC results for these markers were compared on mouse spleen tissue treated with neutral buffered formalin (NBF) or ZN with or ZN without antigen retrieval (AR). Whereas CD4 and CD8 were not detected in NBF-treated tissue, all markers were detected in ZN-treated tissue without AR. Thus, the use of ZN treatment for IHC staining can be a good tool for studying immunoreactive lesions in tissues.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Zinc / Inmunohistoquímica / Biomarcadores / Fijación del Tejido / Inmunidad Celular Tipo de estudio: Diagnostic_studies / Guideline Límite: Animals Idioma: En Revista: Toxicol Pathol Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Zinc / Inmunohistoquímica / Biomarcadores / Fijación del Tejido / Inmunidad Celular Tipo de estudio: Diagnostic_studies / Guideline Límite: Animals Idioma: En Revista: Toxicol Pathol Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos