Concurrent measurement of cellular turbidity and hemoglobin to evaluate the antioxidant activity of plants.
Food Chem
; 190: 468-473, 2016 Jan 01.
Article
en En
| MEDLINE
| ID: mdl-26212998
ABSTRACT
In past decades, a multitude of analytical methods for measuring antioxidant activity of plant extracts has been developed. However, when using methods to determine hemoglobin released from human erythrocytes treated with ginger extracts, we found hemoglobin concentrations were significantly higher than in untreated control samples. This suggests in the presence of antioxidants that measuring hemoglobin alone is not sufficient to determine hemolysis. We show concurrent measurement of erythrocyte concentration and hemoglobin is essential in such assays, and describe a new protocol based on simultaneous measurement of cellular turbidity and hemoglobin.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hemoglobinas
/
Extractos Vegetales
/
Antioxidantes
Límite:
Humans
Idioma:
En
Revista:
Food Chem
Año:
2016
Tipo del documento:
Article