In Vitro T-Cell Generation From Adult, Embryonic, and Induced Pluripotent Stem Cells: Many Roads to One Destination.
Stem Cells
; 33(11): 3174-80, 2015 Nov.
Article
en En
| MEDLINE
| ID: mdl-26227158
ABSTRACT
T lymphocytes are critical mediators of the adaptive immune system and have the capacity to serve as therapeutic agents in the areas of transplant and cancer immunotherapy. While T cells can be isolated and expanded from patients, T cells derived in vitro from both hematopoietic stem/progenitor cells (HSPCs) and human pluripotent stem cells (hPSCs) offer great potential advantages in generating a self-renewing source of T cells that can be readily genetically modified. T-cell differentiation in vivo is a complex process requiring tightly regulated signals; providing the correct signals in vitro to induce T-cell lineage commitment followed by their development into mature, functional, single positive T cells, is similarly complex. In this review, we discuss current methods for the in vitro derivation of T cells from murine and human HSPCs and hPSCs that use feeder-cell and feeder-cell-free systems. Furthermore, we explore their potential for adoption for use in T-cell-based therapies.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Linfocitos T
/
Diferenciación Celular
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Células Madre Adultas
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Células Madre Embrionarias
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Células Madre Pluripotentes Inducidas
Límite:
Adult
/
Animals
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Humans
Idioma:
En
Revista:
Stem Cells
Año:
2015
Tipo del documento:
Article
País de afiliación:
Estados Unidos