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Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil.
Dantas, Nayanne Gama Teixeira; Suffys, Phillip Noel; Carvalho, Wânia da Silva; Gomes, Harrison Magdinier; de Almeida, Isabela Neves; de Assis, Lida Jouca; Augusto, Claudio José; Gomgnimbou, Michel Kireopori; Refregier, Guislaine; Sola, Christophe; de Miranda, Silvana Spíndola.
Afiliación
  • Dantas NG; Post-Graduate Program in Infectious Diseases and Tropical Medicine, Department of Internal medicine, Faculty of Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil. ngtdantas@gmail.com.
  • Suffys PN; Laboratory of Molecular Biology Applied to Mycobacteria, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil. psuffys@gmail.com.
  • Carvalho Wda S; Laboratory of Molecular Biology and Public Health, Department of Social Pharmacy, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil. waniasilvacarvalho@gmail.com.
  • Gomes HM; Laboratory of Molecular Biology Applied to Mycobacteria, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil. magdinier@gmail.com.
  • de Almeida IN; Post-Graduate Program in Infectious Diseases and Tropical Medicine, Department of Internal medicine, Faculty of Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil. isabelanalmeida@gmail.com.
  • de Assis LJ; Laboratory of Molecular Biology and Public Health, Department of Social Pharmacy, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil. lidajouca@hotmail.com.
  • Augusto CJ; Ezequiel Dias Foundation, Belo Horizonte, Brazil. augustocj@gmail.com.
  • Gomgnimbou MK; Institut for Integrative Cell Biology, I2BC, UMR9198 CEA-CNRS-UPSaclay, Orsay, France. gomikir@yahoo.fr.
  • Refregier G; Centre Muraz, Bobo-Dioulasso, Burkina Faso. gomikir@yahoo.fr.
  • Sola C; Institut for Integrative Cell Biology, I2BC, UMR9198 CEA-CNRS-UPSaclay, Orsay, France. guislaine.refregier@i2bc.paris-saclay.fr.
  • de Miranda SS; Institut for Integrative Cell Biology, I2BC, UMR9198 CEA-CNRS-UPSaclay, Orsay, France. christophe.sola@i2bc.paris-saclay.fr.
BMC Infect Dis ; 15: 306, 2015 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-26231661
ABSTRACT

BACKGROUND:

We aimed to characterize the genetic diversity of drug-resistant Mycobacterium tuberculosis (MTb) clinical isolates and investigate the molecular epidemiology of multidrug-resistant (MDR) tuberculosis from Minas Gerais State, Brazil.

METHODS:

One hundred and four MTb clinical isolates were assessed by IS6110-RFLP, 24-locus mycobacterial interspersed repetitive units variable-number tandem repeats (MIRU-VNTR), TB-SPRINT (simultaneous spoligotyping and rifampicin-isoniazid drug-resistance mutation analysis) and 3R-SNP-typing (analysis of single-nucleotide polymorphisms in the genes involved in replication, recombination and repair functions).

RESULTS:

Fifty-seven different IS6110-RFLP patterns were found, among which 50 had unique patterns and 17 were grouped into seven clusters. The discriminatory index (Hunter and Gaston, HGDI) for RFLP was 0.9937. Ninety-nine different MIRU-VNTR patterns were found, 95 of which had unique patterns and nine isolates were grouped into four clusters. The major allelic diversity index in the MIRU-VNTR loci ranged from 0.6568 to 0.7789. The global HGDI for MIRU-VNTR was 0.9991. Thirty-two different spoligotyping profiles were found 16 unique patterns (n = 16) and 16 clustered profiles (n = 88). The HGDI for spoligotyping was 0.9009. The spoligotyped clinical isolates were phylogenetically classified into Latin-American Mediterranean (66.34 %), T (14.42 %), Haarlem (5.76 %), X (1.92 %), S (1.92 %) and U (unknown profile; 8.65 %). Among the U isolates, 77.8 % were classified further by 3R-SNP-typing as 44.5 % Haarlem and 33.3 % LAM, while the 22.2 % remaining were not classified. Among the 104 clinical isolates, 86 were identified by TB-SPRINT as MDR, 12 were resistant to rifampicin only, one was resistant to isoniazid only, three were susceptible to both drugs, and two were not successfully amplified by PCR. A total of 42, 28 and eight isolates had mutations in rpoB positions 531, 526 and 516, respectively. Correlating the cluster analysis with the patient data did not suggest recent transmission of MDR-TB.

CONCLUSIONS:

Although our results do not suggest strong transmission of MDR-TB in Minas Gerais (using a classical 100 % MDR-TB identical isolates cluster definition), use of a smoother cluster definition (>85 % similarity) does not allow us to fully eliminate this possibility; hence, around 20-30 % of the isolates we analyzed might be MDR-TB transmission cases.
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Texto completo: Disponible Colección: Bases de datos internacionales Contexto: OBJETIVO DE DESARROLLO SOSTENIBLE 3 - ODS3 / Enfermedades desatendidas Tema en salud: Meta 3.3: Poner fin a las enfermedades desatendidas y detener enfermedades transmisibles / Tuberculosis Base de datos: MEDLINE Asunto principal: Variación Genética / Tuberculosis Resistente a Múltiples Medicamentos / Mycobacterium tuberculosis Tipo de estudio: Estudio de tamizaje Límite: Humanos País/Región como asunto: America del Sur / Brasil Idioma: Inglés Revista: BMC Infect Dis Asunto de la revista: Enfermedades Transmisibles Año: 2015 Tipo del documento: Artículo País de afiliación: Brasil