Antibiotic resistance marker genes as environmental pollutants in GMO-pristine agricultural soils in Austria.
Environ Pollut
; 206: 342-51, 2015 Nov.
Article
en En
| MEDLINE
| ID: mdl-26232739
ABSTRACT
Antibiotic resistance genes may be considered as environmental pollutants if anthropogenic emission and manipulations increase their prevalence above usually occurring background levels. The prevalence of aph(3')-IIa/nptII and aph(3')-IIIa/nptIII - frequent marker genes in plant biotechnology conferring resistance to certain aminoglycosides - was determined in Austrian soils from 100 maize and potato fields not yet exposed to but eligible for GMO crop cultivation. Total soil DNA extracts were analysed by nptII/nptIII-specific TaqMan real time PCR. Of all fields 6% were positive for nptII (median 150 copies/g soil; range 31-856) and 85% for nptIII (1190 copies/g soil; 13-61600). The copy-number deduced prevalence of nptIII carriers was 14-fold higher compared to nptII. Of the cultivable kanamycin-resistant soil bacteria 1.8% (95% confidence interval 0-3.3%) were positive for nptIII, none for nptII (0-0.8%). The nptII-load of the studied soils was low rendering nptII a typical candidate as environmental pollutant upon anthropogenic release into these ecosystems.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Suelo
/
Microbiología del Suelo
/
Contaminantes del Suelo
/
Productos Agrícolas
/
Farmacorresistencia Bacteriana
/
Genes Bacterianos
/
Antibacterianos
Tipo de estudio:
Risk_factors_studies
País/Región como asunto:
Europa
Idioma:
En
Revista:
Environ Pollut
Asunto de la revista:
SAUDE AMBIENTAL
Año:
2015
Tipo del documento:
Article