Isolation of Pure Nuclei Using a Sucrose Method.
Cold Spring Harb Protoc
; 2015(8): 773-6, 2015 Aug 03.
Article
en En
| MEDLINE
| ID: mdl-26240404
ABSTRACT
This protocol describes a modified version of a widely used method to isolate nuclei from tissue culture cells. It involves mechanical homogenization of cells in isotonic sucrose, followed by velocity centrifugation of nuclei through a denser layer of sucrose. This method, which yields highly pure and intact nuclei, can be optimized for use in various types of tissues and cells. Limitations of the method, alternative options for homogenization, and recommendations for the use of detergents are discussed.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sacarosa
/
Fraccionamiento Celular
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Núcleo Celular
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Centrifugación
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Técnicas Histológicas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Cold Spring Harb Protoc
Año:
2015
Tipo del documento:
Article