Difference in microchip electrophoretic mobility between partially and fully PEGylated poly(amidoamine) dendrimers.
Anal Biochem
; 488: 9-11, 2015 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-26253023
ABSTRACT
The objective of this study was to investigate the difference in electrophoretic mobility between partially and fully poly(ethylene glycol)-conjugated poly(amidoamine) dendrimers (part-PEG-PAMAM and full-PEG-PAMAM, respectively) using a microchip capillary gel electrophoresis (MCGE). While MCGE allowed size-based separation of PEG-PAMAMs prepared with monomethoxy PEG-nitrophenyl carbonate, full-PEG-PAMAMs migrated slower than part-PEG-PAMAMs that were similar in size or larger. When the measured molecular weights obtained from MCGE analysis and the calculated molecular weights were plotted, each part-PEG-PAMAM and full-PEG-PAMAM showed correlation coefficients greater than 0.98. This study indicates that MCGE would be useful for characterizing PEG-PAMAMs with different PEGylation degrees.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Poliaminas
/
Polietilenglicoles
/
Dendrímeros
País/Región como asunto:
Asia
Idioma:
En
Revista:
Anal Biochem
Año:
2015
Tipo del documento:
Article