Bee venom phospholipase A2 as a membrane-binding vector for cell surface display or internalization of soluble proteins.
Toxicon
; 116: 56-62, 2016 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-26253725
ABSTRACT
We showed that bee venom phospholipase A2 can be used as a membrane-binding vector to anchor to the surface of cells a soluble protein fused to its C-terminus. ZZ, a two-domain derivative of staphylococcal protein A capable of binding constant regions of antibodies was fused to the C-terminus of the phospholipase or to a mutant devoid of enzymatic activity. The fusion proteins bound to the surface of cells and could themselves bind IgGs. Their fate depended on the cell type to which they bound. On the A431 carcinoma cell line the proteins remained exposed on the cell surface. In contrast, on human dendritic cells the proteins were internalized into early endosomes.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Venenos de Abeja
/
Membrana Celular
/
Fosfolipasas A2
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Toxicon
Año:
2016
Tipo del documento:
Article
País de afiliación:
Francia