CRISPR mediated somatic cell genome engineering in the chicken.
Dev Biol
; 407(1): 68-74, 2015 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-26277216
ABSTRACT
Gene-targeted knockout technologies are invaluable tools for understanding the functions of genes in vivo. CRISPR/Cas9 system of RNA-guided genome editing is revolutionizing genetics research in a wide spectrum of organisms. Here, we combined CRISPR with in vivo electroporation in the chicken embryo to efficiently target the transcription factor PAX7 in tissues of the developing embryo. This approach generated mosaic genetic mutations within a wild-type cellular background. This series of proof-of-principle experiments indicate that in vivo CRISPR-mediated cell genome engineering is an effective method to achieve gene loss-of-function in the tissues of the chicken embryo and it completes the growing genetic toolbox to study the molecular mechanisms regulating development in this important animal model.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ingeniería Genética
/
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Dev Biol
Año:
2015
Tipo del documento:
Article
País de afiliación:
Australia