A Dutch nationwide evaluation of serological assays for detection of Borrelia antibodies in clinically well-defined patients.
Diagn Microbiol Infect Dis
; 83(3): 222-8, 2015 Nov.
Article
en En
| MEDLINE
| ID: mdl-26286381
ABSTRACT
Numerous tests for the detection of antibodies against Borrelia burgdorferi are commercially available. Manufacturer-derived data invariably report a high sensitivity and specificity, but comparative studies demonstrate large differences in clinical practice, especially with regard to specificity. We retrospectively collected data from validation studies for B. burgdorferi antibody assays from 8 laboratories in the Netherlands. The total number of samples was 809. Samples were selected based on clinical and laboratory parameters. We included samples from patients with erythema migrans, acrodermatitis chronicum atrophicans, and neuroborreliosis; cross-reactivity controls; and healthy controls. Data are presented from 10 enzyme-linked immunosorbent assays and 5 immunoblots. For manifestations of B. burgdorferi infection with short disease duration, the positivity rate of the assays varied significantly. In patients with long disease duration, the positivity rate differed only marginally. In cross-reactivity controls, there was significant variation in the reactivity rate. The majority of false-positive reactions are of the IgM isotype.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedad de Lyme
/
Pruebas Serológicas
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Borrelia burgdorferi
/
Anticuerpos Antibacterianos
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
/
Observational_studies
Límite:
Humans
País/Región como asunto:
Europa
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Año:
2015
Tipo del documento:
Article
Pais de publicación:
EEUU
/
ESTADOS UNIDOS
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ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA