New assay for Gardnerella vaginalis loads correlates with Nugent scores and has potential in the diagnosis of bacterial vaginosis.
J Med Microbiol
; 64(9): 978-984, 2015 Sep.
Article
en En
| MEDLINE
| ID: mdl-26296660
ABSTRACT
Gardnerella vaginalis is a Gram-variable anaerobic bacterium present in 100% of women with bacterial vaginosis (BV). BV is a complex polymicrobial condition with no single causative agent. The current laboratory detection method for BV relies on a Gram-stain Nugent score to estimate the quantity of different bacterial morphotypes in the vaginal micro flora. Whilst the Nugent score can distinguish between women with and without BV, a significant proportion are categorized as intermediate, which fails to differentiate a normal from an abnormal vaginal micro flora. A singleplex G. vaginalis TaqMan real-time quantitative PCR (qPCR) assay was developed and compared with the 'gold standard' Nugent score. Detection and quantification of G. vaginalis was performed on vaginal specimens with positive, negative and intermediate Nugent scores. The G. vaginalis qPCR assay demonstrated high analytical specificity against a broad microbial panel and analytical sensitivity down to 3.1 × 10(4) copies ml(-1). There was a significantly higher G. vaginalis load in women with BV compared with intermediate and non-BV women (P value = 5.1 × 10(-14)). All Nugent scores in keeping with BV had qPCR loads of ≥ 10(7) copies ml(-1). Among the 24 undefined women (11.8%) in the study with an intermediate flora, 14 (58.3%) had a G. vaginalis load of ≥ 10(7) copies ml(-1). In this study a threshold of 107 copies ml(-1) had positive and negative predictive values of 57.1 and 100% for BV; the high qPCR loads among the intermediate Nugent scores suggest the need for a new approach in classifying BV and the potential for qPCR to play a role.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Gardnerella vaginalis
/
Vaginosis Bacteriana
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
J Med Microbiol
Año:
2015
Tipo del documento:
Article
País de afiliación:
Reino Unido
Pais de publicación:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM