ROS production in homogenate from the body wall of sea cucumber Stichopus japonicus under UVA irradiation: ESR spin-trapping study.
Food Chem
; 192: 358-62, 2016 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-26304359
ABSTRACT
Sea cucumber Stichopus japonicus (S. japonicus) shows a strong ability of autolysis, which leads to severe deterioration in sea cucumber quality during processing and storage. In this study, to further characterize the mechanism of sea cucumber autolysis, hydroxyl radical production induced by ultraviolet A (UVA) irradiation was investigated. Homogenate from the body wall of S. japonicas was prepared and subjected to UVA irradiation at room temperature. Electron Spin Resonance (ESR) spectra of the treated samples were subsequently recorded. The results showed that hydroxyl radicals (OH) became more abundant while the time of UVA treatment and the homogenate concentration were increased. Addition of superoxide dismutase (SOD), catalase, EDTA, desferal, NaN3 and D2O to the homogenate samples led to different degrees of inhibition on OH production. Metal cations and pH also showed different effects on OH production. These results indicated that OH was produced in the homogenate with a possible pathway as follows O2(-) â H2O2 â OH, suggesting that OH might be a critical factor in UVA-induced S. japonicus autolysis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Rayos Ultravioleta
/
Especies Reactivas de Oxígeno
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Stichopus
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Análisis de los Alimentos
Límite:
Animals
Idioma:
En
Revista:
Food Chem
Año:
2016
Tipo del documento:
Article