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Development and validation of an inexpensive and efficient method for the extraction of Theileria orientalis DNA from blood.
Bogema, D R; Fell, S A; O'Rourke, B A; Collins, D; Eamens, G J; Jenkins, C.
Afiliación
  • Bogema DR; The ithree institute, University of Technology Sydney, Ultimo, NSW 2007, Australia; Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW 2568, Australia.
  • Fell SA; Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW 2568, Australia.
  • O'Rourke BA; Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW 2568, Australia.
  • Collins D; Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW 2568, Australia.
  • Eamens GJ; Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW 2568, Australia.
  • Jenkins C; Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW 2568, Australia. Electronic address: cheryl.jenkins@dpi.nsw.gov.au.
Vet Parasitol ; 212(3-4): 379-81, 2015 Sep 15.
Article en En | MEDLINE | ID: mdl-26319198
Theileria orientalis is an emerging bovine pathogen in Australasia. PCR-based detection methods for this parasite are sensitive but relatively expensive, partly due to costs associated with DNA extraction. An inexpensive and efficient technique was developed for the extraction of T. orientalis DNA from blood based on hypotonic erythrocyte lysis and detergent-proteinase K treatment (DPK method). The DPK method compares favourably to a commercial extraction kit when paired with a T. orientalis multiplex qPCR.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Theileriosis / ADN Protozoario / Theileria Límite: Animals Idioma: En Revista: Vet Parasitol Año: 2015 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Theileriosis / ADN Protozoario / Theileria Límite: Animals Idioma: En Revista: Vet Parasitol Año: 2015 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos