Detection of the dinoflagellate, Cochlodinium polykrikoides, that forms algal blooms using sandwich hybridization integrated with nuclease protection assay.
Biotechnol Lett
; 38(1): 57-63, 2016 Jan.
Article
en En
| MEDLINE
| ID: mdl-26334938
ABSTRACT
OBJECTIVES:
To detect Cochlodinum polykrikoides in long-term monitoring and high-throughput sampling projects using an integrated sandwich hybridization and nuclease protection assay (NPA-SH).RESULTS:
The specificity of the probes was verified with individual and mixed cultures as well as field collection, and the quantity of C. polykrikoides determined by NPA-SH analysis showed a good correlation with that determined by cell-counting with a light microscope. In addition a standard curve for C. polykrikoides was established to represent the correlation between optical absorbance in the NPA-SH assay and cell density.CONCLUSIONS:
This approach provides an efficient alternative to traditional, morphology-based methods for the rapid identification and quantification of harmful algal species and could be used to monitor phytoplankton in field surveys.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Dinoflagelados
/
Ensayos de Protección de Nucleasas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Biotechnol Lett
Año:
2016
Tipo del documento:
Article