Genomic characterization and sequence diversity of the ß2-microglobulin gene in the miiuy croaker, Miichthys miiuy.
Genet Mol Res
; 14(3): 10249-57, 2015 Aug 28.
Article
en En
| MEDLINE
| ID: mdl-26345962
ABSTRACT
ß2-Microglobulin (ß2m) is related to major histocompatibility complex class I alpha chains, and forms cell-surface glycoproteins that mediate a variety of functions in immune defense. In general, ß2m has no isoforms and is not polymorphic in higher vertebrates, but polymorphisms between different alleles have been found in some fish species. In this study, full-length ß2m cDNA and genomic sequences were cloned from the miiuy croaker (Miichthys miiuy). The miiuy croaker ß2m gene shares many of the same characteristics as other fish species. Three exons and two introns were identified in the miiuy croaker ß2m gene; these genomic structural features are similar to those present in other fish. The deduced ß2m amino acid sequence exhibited 34.7-90.1% identity with mammal and teleost ß2m amino acid sequences. Sequence polymorphism analysis in six individuals identified three alleles that encoded two proteins, confirming that ß2m polymorphisms exist in this species. Phylogenetic analysis elucidated the evolutionary history of the ß2m protein among warm-blooded vertebrates and bony fish.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Variación Genética
/
Perciformes
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Microglobulina beta-2
/
Genoma
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Genet Mol Res
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2015
Tipo del documento:
Article
País de afiliación:
China