A Kelch Domain-Containing F-Box Coding Gene Negatively Regulates Flavonoid Accumulation in Muskmelon.
Plant Physiol
; 169(3): 1714-26, 2015 Nov.
Article
en En
| MEDLINE
| ID: mdl-26358418
ABSTRACT
The flavonoids are phenylpropanoid-derived metabolites that are ubiquitous in plants, playing many roles in growth and development. Recently, we observed that fruit rinds of yellow casaba muskmelons (Cucumis melo 'Inodorous Group') accumulate naringenin chalcone, a yellow flavonoid pigment. With RNA-sequencing analysis of bulked segregants representing the tails of a population segregating for naringenin chalcone accumulation followed by fine mapping and genetic transformation, we identified a Kelch domain-containing F-box protein coding (CmKFB) gene that, when expressed, negatively regulates naringenin chalcone accumulation. Additional metabolite analysis indicated that downstream flavonoids are accumulated together with naringenin chalcone, whereas CmKFB expression diverts the biochemical flux toward coumarins and general phenylpropanoids. These results show that CmKFB functions as a posttranscriptional regulator that diverts flavonoid metabolic flux.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Flavonoides
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Regulación de la Expresión Génica de las Plantas
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Cucumis melo
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Proteínas F-Box
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Chalconas
Idioma:
En
Revista:
Plant Physiol
Año:
2015
Tipo del documento:
Article