Unraveling of cross talk between Ca(2+) and ROS regulating enzymes in Anabaena 7120 and ntcA mutant.
J Basic Microbiol
; 56(7): 762-78, 2016 Jul.
Article
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| MEDLINE
| ID: mdl-26374944
ABSTRACT
In order to understand a cross talk between Ca(2+) and ROS regulating enzymes and the possible involvement of ntcA gene, Anabaena sp. PCC 7120 and its derivative ntcA mutant grown in varied levels of calcium chloride (0, 1, 10, and 100 mM) have been investigated. Scanning Electron Microscopy showed abnormal structure formation at high calcium concentration (100 mM) both in wild type and mutant. Fv /Fm values suggested that 100 mM calcium concentration was detrimental for photosynthetic apparatus. SOD, catalase, APX, GR, and peroxidase activity were found to be maximum for 100 mM and minimum for 1 mM of exogenously supplied calcium salt. NADPH contents were higher for wild type than mutant. RAPD-PCR and SDS-PAGE analysis revealed a difference in DNA as well as proteome pattern with changes in calcium chloride regime. Prominent bands of approximately 70, 33, 21, and 14 kDa expressed in the wild type served as the marker polypeptide bands under calcium supplementation. Results suggest that higher levels of calcium ion disturb the cellular homeostasis generating ROS, thereby inducing enhanced levels of antioxidative enzymes. Further, data also suggests possible involvement of ntcA gene in cross talk between calcium ion and ROS regulating enzymes.
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Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
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Cloruro de Calcio
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Anabaena
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Especies Reactivas de Oxígeno
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Proteínas PII Reguladoras del Nitrógeno
Idioma:
En
Revista:
J Basic Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
India