Tolerance response to in situ ammonia stress in a pilot-scale anaerobic digestion reactor for alleviating ammonia inhibition.
Bioresour Technol
; 198: 372-9, 2015 Dec.
Article
en En
| MEDLINE
| ID: mdl-26409107
ABSTRACT
The anaerobic digestion (AD) of protein-rich substrates is generally inhibited by ammonia. In this study, ammonia-tolerant acclimation was exposed to a stepwise in situ ammonia stress during the continuous AD of solid residual kitchen waste by using a continuous stirred tank reactor with a 50 L active volume. The reactor worked well during the acclimation process, with an average daily biogas production of 58 L/d, an effluent soluble chemical oxygen demand of 7238 mg/L, a volatile fatty acid (VFA) content of 578 mg/L, and a VFA/alkalinity ratio of less than 0.4. Moreover, ammonia stress enhanced the activity of Coenzyme F420. The results of high-throughput 16S rDNA sequencing showed that ammonia stress increased the relative abundance of Firmicutes bacteria and hydrogenotrophic methanogens but decreased the abundance of acetotrophic methanogens. This microbial community shift was proposed to be an in situ response strategy for ammonia stress adaptation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aguas del Alcantarillado
/
Ácidos Grasos Volátiles
/
Amoníaco
/
Anaerobiosis
Idioma:
En
Revista:
Bioresour Technol
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2015
Tipo del documento:
Article
País de afiliación:
China