Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease.
Nat Biotechnol
; 33(11): 1159-61, 2015 Nov.
Article
en En
| MEDLINE
| ID: mdl-26436575
ABSTRACT
We have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. These sgRNAs, with 14- to 15-bp target sequences and MS2 binding loops, can activate gene expression using an active Streptococcus pyogenes Cas9 nuclease, without inducing double-stranded breaks. We use these 'dead RNAs' to perform orthogonal gene knockout and transcriptional activation in human cells.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
ARN Guía de Kinetoplastida
/
Endonucleasas
/
Técnicas de Inactivación de Genes
Límite:
Humans
Idioma:
En
Revista:
Nat Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2015
Tipo del documento:
Article