Biosynthesis of Novel Glucosides Geldanamycin Analogs by Enzymatic Synthesis.
J Microbiol Biotechnol
; 26(1): 56-60, 2016 Jan.
Article
en En
| MEDLINE
| ID: mdl-26464380
ABSTRACT
Two new glucosides (1 and 2) of geldanamycin (GA) analogs were obtained from in vitro glycosylation by UDP-glycosyltransferase (YjiC). Based on spectroscopic (HR-ESI-MS, 1D, and 2D-NMR) analyses, the glucosides were elucidated as 4,5-dihydro-7-O-descarbamoyl-7- hydroxyl GA-7-O-ß-D-glucoside (1) and ACDL3172-18-O-ß-D-glucoside (2). Furthermore, the water solubility of compounds 1 and 2 was about 215.2 and 90.7 times higher respectively, than that of the substrates. Among compounds 1-4, only 3 showed weak antiproliferative activity against four human tumor cell lines MDA-MB-231, SMMC7721, HepG2, and SW480 (IC50 13.6, 15.1, 31.8, and 22.7 micrometer, respectively).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Benzoquinonas
/
Glicosiltransferasas
/
Lactamas Macrocíclicas
/
Glucósidos
Límite:
Humans
Idioma:
En
Revista:
J Microbiol Biotechnol
Año:
2016
Tipo del documento:
Article