The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models.
BMC Cancer
; 15: 729, 2015 Oct 17.
Article
en En
| MEDLINE
| ID: mdl-26474850
ABSTRACT
BACKGROUND:
The GABARAP family members (GABARAP, GABARAPL1/GEC1 and GABARAPL2 /GATE-16) are involved in the intracellular transport of receptors and the autophagy pathway. We previously reported that GABARAPL1 expression was frequently downregulated in cancer cells while a high GABARAPL1 expression is a good prognosis marker for patients with lymph node-positive breast cancer.METHODS:
In this study, we asked using qRT-PCR, western blotting and epigenetic quantification whether the expression of the GABARAP family was regulated in breast cancer by epigenetic modifications.RESULTS:
Our data demonstrated that a specific decrease of GABARAPL1 expression in breast cancers was associated with both DNA methylation and histone deacetylation and that CREB-1 recruitment on GABARAPL1 promoter was required for GABARAPL1 expression.CONCLUSIONS:
Our work strongly suggests that epigenetic inhibitors and CREB-1 modulators may be used in the future to regulate autophagy in breast cancer cells.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de la Mama
/
Proteína de Unión a Elemento de Respuesta al AMP Cíclico
/
Metilación de ADN
/
Proteínas Adaptadoras Transductoras de Señales
/
Proteínas Asociadas a Microtúbulos
Límite:
Female
/
Humans
Idioma:
En
Revista:
BMC Cancer
Asunto de la revista:
NEOPLASIAS
Año:
2015
Tipo del documento:
Article
País de afiliación:
Francia