When the Label Matters: Adsorption of Labeled and Unlabeled Proteins on Charged Surfaces.
Nano Lett
; 15(11): 7508-13, 2015 Nov 11.
Article
en En
| MEDLINE
| ID: mdl-26491986
ABSTRACT
Fluorescent labels are often attached to proteins to monitor binding and adsorption processes. Docking simulations for native hen egg white lysozyme (HEWL) and HEWL labeled with fluorescein isothiocyanate show that these adsorb differently on charged surfaces. Attachment of even a small label can significantly change the interaction properties of a protein. Thus, the results of experiments with fluorescently labeled proteins should be interpreted by modeling the structures and computing the interaction properties of both labeled and unlabeled species.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Coloración y Etiquetado
/
Muramidasa
/
Adsorción
/
Colorantes Fluorescentes
Límite:
Animals
Idioma:
En
Revista:
Nano Lett
Año:
2015
Tipo del documento:
Article
País de afiliación:
Alemania