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Mechanism of two-photon excited hemoglobin fluorescence emission.
Sun, Qiqi; Zheng, Wei; Wang, Jiannong; Luo, Yi; Qu, Jianan Y.
Afiliación
  • Sun Q; Hong Kong University of Science and Technology, Department of Electronic and Computer Engineering, Clear Water Bay, Kowloon, Hong Kong SAR, China.
  • Zheng W; Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
  • Wang J; Hong Kong University of Science and Technology, Department of Physics, Clear Water Bay, Kowloon, Hong Kong SAR, China.
  • Luo Y; University of Science and Technology of China, Bio-X Division, Hefei National Laboratory for Physical Science at the Microscale, Hefei, Anhui, China.
  • Qu JY; Hong Kong University of Science and Technology, Department of Electronic and Computer Engineering, Clear Water Bay, Kowloon, Hong Kong SAR, ChinaeHong Kong University of Science and Technology, Center of Systems Biology and Human Health, School of Science.
J Biomed Opt ; 20(10): 105014, 2015 Oct.
Article en En | MEDLINE | ID: mdl-26506468
Hemoglobin, one of the most important proteins in the human body, is composed of "heme" groups (iron-containing rings) and "globins" (proteins). We investigate the two-photon excited fluorescence of hemoglobin and its subunit components (heme and globin). We measure the hemoglobin fluorescence lifetime by using a streak camera of ps resolution and confirm that its lifetime is in femtosecond scale. In the study of the fluorescence properties of heme and globin, the experimental results reveal that heme is the sole fluorophore of hemoglobin. Hemoglobin fluorescence can be effectively excited only via two-photon process, because heme has a centrosymmetric molecular structure and two-photon allowed transition is forbidden for single-photon process and vice versa due to the Laporte parity selection rule.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hemoglobinas / Microscopía de Fluorescencia por Excitación Multifotónica / Imagen Molecular / Microscopía Fluorescente / Modelos Químicos Límite: Animals Idioma: En Revista: J Biomed Opt Asunto de la revista: ENGENHARIA BIOMEDICA / OFTALMOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hemoglobinas / Microscopía de Fluorescencia por Excitación Multifotónica / Imagen Molecular / Microscopía Fluorescente / Modelos Químicos Límite: Animals Idioma: En Revista: J Biomed Opt Asunto de la revista: ENGENHARIA BIOMEDICA / OFTALMOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos