Point Mutation Ile137-Met Near Surface Conferred Psychrophilic Behaviour and Improved Catalytic Efficiency to Bacillus Lipase of 1.4 Subfamily.

Goomber, Shelly; Kumar, Arbind; Singh, Ranvir; Kaur, Jagdeep

Goomber, Shelly; Kumar, Arbind; Singh, Ranvir; Kaur, Jagdeep.

Afiliación

Goomber S; Department of Biotechnology, Sector 25, Panjab University, Chandigarh, 160014, India.
Kumar A; Department of Biotechnology, Sector 25, Panjab University, Chandigarh, 160014, India.
Singh R; National Centre for Human Genome Studies and Research, Panjab University, Chandigarh, 160014, India.
Kaur J; Department of Biotechnology, Sector 25, Panjab University, Chandigarh, 160014, India. jagsekhon@yahoo.com.

Appl Biochem Biotechnol ; 178(4): 753-65, 2016 Feb.

Article en En | MEDLINE | ID: mdl-26520838

Asunto(s)

Bacillus subtilis/enzimología; Isoleucina/genética; Lipasa/metabolismo; Metionina/genética; Mutación Puntual; Biocatálisis; Clonación Molecular; Cinética; Lipasa/genética; Lipasa/aislamiento & purificación; Modelos Moleculares

Palabras clave

Bacillus subtilis lipase; Homolog modelling; Laboratory evolution; Overlap site-directed mutagenesis; Psychrophilic

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus subtilis / Mutación Puntual / Isoleucina / Lipasa / Metionina Tipo de estudio: Prognostic_studies Idioma: En Revista: Appl Biochem Biotechnol Año: 2016 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos

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