HPLC determination of α-ketoglutaramate [5-amino-2,5-dioxopentanoate] in biological samples.
Anal Biochem
; 494: 52-4, 2016 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-26576832
ABSTRACT
α-Ketoglutaramate is an important glutamine metabolite in mammals, plants, and many bacteria. It is also a nicotine metabolite in certain bacteria. Previously published methods for the determination of α-ketoglutaramate in biological samples have considerable drawbacks. Here, we describe a relatively simple high-performance liquid chromatography (HPLC)-based method for measurement of α-ketoglutaramate in plasma and deproteinized tissues that overcomes these drawbacks. Concentrations of α-ketoglutaramate in normal rat liver, kidney, brain, and plasma were found to be approximately 216, 13, 6, and 19 µM, respectively. The HPLC method should be useful for studying the role of α-ketoglutaramate in eukaryotic glutamine metabolism and in bacterial nicotine metabolism.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas de Química Analítica
/
Cromatografía Líquida de Alta Presión
/
Ácidos Cetoglutáricos
Límite:
Animals
Idioma:
En
Revista:
Anal Biochem
Año:
2016
Tipo del documento:
Article
País de afiliación:
Rusia