[Analysis of phenotype resistance of clinical strains of Escherichia coli]. / Analýza fenotypu rezistencie klinického kmena Escherichia coli.

ABSTRACT

The multiplasmid clinical E. coli KL4 strain isolated from urine of a patient was examined for resistance to antibacterial substances and the number of plasmids. The distribution of resistance genes to antibiotics in different plasmids was assessed. All assessed resistances were transmitted by the mechanism of bacterial conjugation. By means of conjugation of the clinical E. coli strain KL4 and E. coli DH1, transformation of the DH1 strain by plasmid DNA and electrophoretic analysis two R plasmids were identified. The largest plasmid carries the gene for resistance against tetracycline and it is conjugative. Another three plasmids were mobilized by this plasmid during conjugation. The largest among them codes resistance against ampicillin, azlocillin and ticarcillin. From the total of five plasmids of the KL4 strain three were cryptic with regard to the resistance phenotype.