Effects of miR590 on oxLDLinduced endothelial cell apoptosis: Roles of p53 and NFκB.
Mol Med Rep
; 13(1): 867-73, 2016 Jan.
Article
en En
| MEDLINE
| ID: mdl-26648441
ABSTRACT
Oxidized lowdensity lipoprotein (oxLDL)induced endothelial cell apoptosis is considered to be important in atherogenesis. MicroRNA (miR)590 has been reported to inhibit oxLDLinduced endothelial cell apoptosis. However, the mechanism underlying the inhibition of oxLDLinduced endothelial cell apoptosis by miR590 remains to be elucidated. In the present study, the expression levels of miR590 were quantified using reverse transcriptionquantitative polymerase chain reaction analysis. Cell apoptosis was investigated using Hoechst staining and flow cytometry, and cell viability was measured using an MTS method. The protein expression levels of p53, B cell lymphoma 2 (Bcl2), Bcl2associated X protein (Bax), caspase3, lectinlike lowdensity lipoprotein receptor 1 (LOX1), p38 mitogenactivated protein kinase (MAPK) and nuclear factor (NF)κB were quantified using western blot analyses. The results of the present study showed that oxLDL treatment inhibited the expression levels of miR590 in a timedependent and concentrationdependent manner. The overexpression of miR590 inhibited oxLDLinduced endothelial cell apoptosis, expression of p53 and Bax, reduction of Bcl2 and activation of caspase3. miR590 also inhibited the oxLDLinduced upregulation of the expression of LOX1, overproduction of reactive oxygen species (ROS), phosphoryation of p38MAPK and translocation of NFκB. These findings demonstrated the antiapoptotic effects of miR590 in oxLDLtreated endothelial cells, with the mechanisms underlying the effects of miR590 involved, in part, in the LOX1ROSp38MAPKNFκB signaling cascade and the p53Bcl2/Baxcaspase3 signaling pathway. The present study may provide novel insights into the protective properties of miR590 in preventing atherosclerosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
FN-kappa B
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Proteína p53 Supresora de Tumor
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Apoptosis
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MicroARNs
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Células Endoteliales de la Vena Umbilical Humana
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Lipoproteínas LDL
Límite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Año:
2016
Tipo del documento:
Article