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Leukocyte telomere shortening in grown-up patients with congenital heart disease.
Vecoli, Cecilia; Borghini, Andrea; Foffa, Ilenia; Ait-Ali, Lamia; Picano, Eugenio; Andreassi, Maria Grazia.
Afiliación
  • Vecoli C; CNR, Institute of Clinical Physiology, Pisa, Italy.
  • Borghini A; CNR, Institute of Clinical Physiology, Pisa, Italy.
  • Foffa I; CNR, Institute of Clinical Physiology, Massa, Italy.
  • Ait-Ali L; CNR, Institute of Clinical Physiology, Massa, Italy.
  • Picano E; CNR, Institute of Clinical Physiology, Pisa, Italy.
  • Andreassi MG; CNR, Institute of Clinical Physiology, Pisa, Italy. Electronic address: mariagrazia.andreassi@ifc.cnr.it.
Int J Cardiol ; 204: 17-22, 2016 Feb 01.
Article en En | MEDLINE | ID: mdl-26655528
BACKGROUND/OBJECTIVES: Children with congenital heart disease are exposed by repeated imaging to ionizing radiation, which may have important implications for lifetime health risks. Leukocyte telomere length (LTL), a reliable biomarker of genomic instability, is associated with increased risk of cancer and cardiovascular disease. We investigated LTL in grown-up patients with CHD (GUCHs) and a positive history of medical radiation exposure as well as the influence of functional polymorphisms of genes involved in DNA repair. METHODS: A group of 50 GUCH patients (26 males; age 25.2 ± 9.0 years) and 50 healthy age/gender-matched subjects (20 males; 27.0 ± 3.1 years) were enrolled. In GUCH patients, the cumulative exposure was estimated as effective dose (ED) in milliSievert. LTL was measured by quantitative RT-PCR. X-ray repair cross complementing-1 (XRCC1) and X-ray repair cross complementing-3 (XRCC3) SNPs (XRCC1Arg399Gln, XRCC1Arg194Tr and XRCC3 Thr241Met) were evaluated. RESULTS: GUCHs showed significantly shorter LTL compared with controls (1.0 ± 0.3 vs 1.3 ± 0.4, p = 0.001). A significant inverse correlation between LTL and cumulative radiological ED was observed (r = -0.34, p = 0.03). Patients with Thr/Met XRCC3 or Met/Met XRCC3 genotypes were significantly associated with a significantly shorter LTL compared with wild-type genotype (p = 0.01 for Thr/Met and p = 0.008 for Met/Met). Carriers of XRCC1 194Trp and XRCC3 241Met alleles presented a significant interaction with cumulative radiation dose exposure for LTL (both p interaction = 0.02). CONCLUSIONS: GUCH patients have LTL shortening, suggesting evidence of early biological aging. Common SNPs in DNA repair genes modify the effects of medical exposure to radiation LTL-related degenerative diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Exposición a la Radiación / Acortamiento del Telómero / Cardiopatías Congénitas / Leucocitos Límite: Adolescent / Adult / Female / Humans / Male Idioma: En Revista: Int J Cardiol Año: 2016 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Exposición a la Radiación / Acortamiento del Telómero / Cardiopatías Congénitas / Leucocitos Límite: Adolescent / Adult / Female / Humans / Male Idioma: En Revista: Int J Cardiol Año: 2016 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Países Bajos