Leukocyte telomere shortening in grown-up patients with congenital heart disease.
Int J Cardiol
; 204: 17-22, 2016 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-26655528
BACKGROUND/OBJECTIVES: Children with congenital heart disease are exposed by repeated imaging to ionizing radiation, which may have important implications for lifetime health risks. Leukocyte telomere length (LTL), a reliable biomarker of genomic instability, is associated with increased risk of cancer and cardiovascular disease. We investigated LTL in grown-up patients with CHD (GUCHs) and a positive history of medical radiation exposure as well as the influence of functional polymorphisms of genes involved in DNA repair. METHODS: A group of 50 GUCH patients (26 males; age 25.2 ± 9.0 years) and 50 healthy age/gender-matched subjects (20 males; 27.0 ± 3.1 years) were enrolled. In GUCH patients, the cumulative exposure was estimated as effective dose (ED) in milliSievert. LTL was measured by quantitative RT-PCR. X-ray repair cross complementing-1 (XRCC1) and X-ray repair cross complementing-3 (XRCC3) SNPs (XRCC1Arg399Gln, XRCC1Arg194Tr and XRCC3 Thr241Met) were evaluated. RESULTS: GUCHs showed significantly shorter LTL compared with controls (1.0 ± 0.3 vs 1.3 ± 0.4, p = 0.001). A significant inverse correlation between LTL and cumulative radiological ED was observed (r = -0.34, p = 0.03). Patients with Thr/Met XRCC3 or Met/Met XRCC3 genotypes were significantly associated with a significantly shorter LTL compared with wild-type genotype (p = 0.01 for Thr/Met and p = 0.008 for Met/Met). Carriers of XRCC1 194Trp and XRCC3 241Met alleles presented a significant interaction with cumulative radiation dose exposure for LTL (both p interaction = 0.02). CONCLUSIONS: GUCH patients have LTL shortening, suggesting evidence of early biological aging. Common SNPs in DNA repair genes modify the effects of medical exposure to radiation LTL-related degenerative diseases.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Exposición a la Radiación
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Acortamiento del Telómero
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Cardiopatías Congénitas
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Leucocitos
Límite:
Adolescent
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Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
Int J Cardiol
Año:
2016
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Países Bajos