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In vivo single-particle imaging of nuclear mRNA export in budding yeast demonstrates an essential role for Mex67p.
Smith, Carlas; Lari, Azra; Derrer, Carina Patrizia; Ouwehand, Anette; Rossouw, Ammeret; Huisman, Maximiliaan; Dange, Thomas; Hopman, Mark; Joseph, Aviva; Zenklusen, Daniel; Weis, Karsten; Grunwald, David; Montpetit, Ben.
Afiliación
  • Smith C; RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605 Department Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605.
  • Lari A; Department of Cell Biology, University of Alberta, T6G 2H7 Edmonton, Alberta, Canada.
  • Derrer CP; Department of Biology, Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland.
  • Ouwehand A; RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605 Department Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605 Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2628 CJ Delf
  • Rossouw A; RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605 Department Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605 Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2628 CJ Delf
  • Huisman M; RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605 Department Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605 Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2628 CJ Delf
  • Dange T; Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2628 CJ Delft, Netherlands.
  • Hopman M; Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2628 CJ Delft, Netherlands.
  • Joseph A; RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605 Department Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605.
  • Zenklusen D; Departement de Biochimie et Medecine Moleculaire, Universite de Montreal, H3T 1J4 Montreal, Quebec, Canada.
  • Weis K; Department of Biology, Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland Department of Cell and Developmental Biology, University of California, Berkeley, Berkeley, CA 94720 ben.montpetit@ualberta.ca david.grunwald@umassmed.edu karsten.weis@bc.biol.ethz.ch.
  • Grunwald D; RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605 Department Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605 Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2628 CJ Delf
  • Montpetit B; Department of Cell Biology, University of Alberta, T6G 2H7 Edmonton, Alberta, Canada ben.montpetit@ualberta.ca david.grunwald@umassmed.edu karsten.weis@bc.biol.ethz.ch.
J Cell Biol ; 211(6): 1121-30, 2015 Dec 21.
Article en En | MEDLINE | ID: mdl-26694837
ABSTRACT
Many messenger RNA export proteins have been identified; yet the spatial and temporal activities of these proteins and how they determine directionality of messenger ribonucleoprotein (mRNP) complex export from the nucleus remain largely undefined. Here, the bacteriophage PP7 RNA-labeling system was used in Saccharomyces cerevisiae to follow single-particle mRNP export events with high spatial precision and temporal resolution. These data reveal that mRNP export, consisting of nuclear docking, transport, and cytoplasmic release from a nuclear pore complex (NPC), is fast (∼ 200 ms) and that upon arrival in the cytoplasm, mRNPs are frequently confined near the nuclear envelope. Mex67p functions as the principal mRNP export receptor in budding yeast. In a mex67-5 mutant, delayed cytoplasmic release from NPCs and retrograde transport of mRNPs was observed. This proves an essential role for Mex67p in cytoplasmic mRNP release and directionality of transport.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / ARN Mensajero / Proteínas Nucleares / Núcleo Celular / Proteínas de Unión al ARN / Proteínas de Transporte Nucleocitoplasmático / Proteínas de Saccharomyces cerevisiae / Transporte de ARN Idioma: En Revista: J Cell Biol Año: 2015 Tipo del documento: Article
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / ARN Mensajero / Proteínas Nucleares / Núcleo Celular / Proteínas de Unión al ARN / Proteínas de Transporte Nucleocitoplasmático / Proteínas de Saccharomyces cerevisiae / Transporte de ARN Idioma: En Revista: J Cell Biol Año: 2015 Tipo del documento: Article