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A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth.
Vissers, Joseph H A; Manning, Samuel A; Kulkarni, Aishwarya; Harvey, Kieran F.
Afiliación
  • Vissers JH; Cell Growth and Proliferation Laboratory, Peter MacCallum Cancer Centre, 7 St Andrews Place, East Melbourne, Melbourne, Victoria 3002, Australia.
  • Manning SA; Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria 3010, Australia.
  • Kulkarni A; Cell Growth and Proliferation Laboratory, Peter MacCallum Cancer Centre, 7 St Andrews Place, East Melbourne, Melbourne, Victoria 3002, Australia.
  • Harvey KF; Department of Pathology, University of Melbourne, Parkville, Victoria 3010, Australia.
Nat Commun ; 7: 10368, 2016 Jan 13.
Article en En | MEDLINE | ID: mdl-26758424
ABSTRACT
Libraries of transgenic Drosophila melanogaster carrying RNA interference (RNAi) constructs have been used extensively to perform large-scale functional genetic screens in vivo. For example, RNAi screens have facilitated the discovery of multiple components of the Hippo pathway, an evolutionarily conserved growth-regulatory network. Here we investigate an important technical limitation with the widely used VDRC KK RNAi collection. We find that approximately 25% of VDRC KK RNAi lines cause false-positive enhancement of the Hippo pathway, owing to ectopic expression of the Tiptop transcription factor. Of relevance to the broader Drosophila community, ectopic tiptop (tio) expression can also cause organ malformations and mask phenotypes such as organ overgrowth. To enhance the use of the VDRC KK RNAi library, we have generated a D. melanogaster strain that will allow researchers to test, in a single cross, whether their genetic screen of interest will be affected by ectopic tio expression.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Animales Modificados Genéticamente / Proteínas Serina-Treonina Quinasas / Proteínas de Drosophila / Interferencia de ARN / Péptidos y Proteínas de Señalización Intracelular / Drosophila melanogaster Límite: Animals Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2016 Tipo del documento: Article País de afiliación: Australia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Animales Modificados Genéticamente / Proteínas Serina-Treonina Quinasas / Proteínas de Drosophila / Interferencia de ARN / Péptidos y Proteínas de Señalización Intracelular / Drosophila melanogaster Límite: Animals Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2016 Tipo del documento: Article País de afiliación: Australia