In vitro activities and detection performances of cefmetazole and flomoxef for extended-spectrum ß-lactamase and plasmid-mediated AmpC ß-lactamase-producing Enterobacteriaceae.
Diagn Microbiol Infect Dis
; 84(4): 322-7, 2016 Apr.
Article
en En
| MEDLINE
| ID: mdl-26782634
ABSTRACT
To investigate the in vitro activities of cephamycins (cefmetazole and flomoxef) for extended-spectrum ß-lactamase (ESBL)- and plasmid-mediated AmpC ß-lactamase (pAmpC)-producing Enterobacteriaceae, a total of 574 third-generation cephalosporin-resistant clinical isolates were collected at a Japanese multicenter study. PCR and sequencing identified 394 isolates with only ESBL genes, 63 isolates with only pAmpC genes, and 6 isolates with both ESBL and pAmpC genes. blaCTX-M types predominated 95.5% of the ESBL genes, and blaCMY-2 predominated 91.3% of the pAmpC genes. The MIC50/90 values of cefmetazole and flomoxef were ≤ 1/4 and ≤ 1/≤ 1 µg/mL for isolates with only ESBL genes, respectively, and 16/>16 and 8/16 µg/mL for isolates with only pAmpC genes, respectively. Flomoxef ≥ 4 µg/mL had the best screening performance for the detection of isolates with pAmpC genes. Flomoxef had better in vitro activities against ESBL-producing Enterobacteriaceae and provided a clearer distinction between ESBL and pAmpC-producing Enterobacteriaceae compared to cefmetazole.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Beta-Lactamasas
/
Cefmetazol
/
Cefalosporinas
/
Resistencia betalactámica
/
Enterobacteriaceae
/
Antibacterianos
Tipo de estudio:
Clinical_trials
/
Diagnostic_studies
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Año:
2016
Tipo del documento:
Article