Purification of biologically active human immunodeficiency virus rev protein from Escherichia coli.
Virology
; 173(1): 335-7, 1989 Nov.
Article
en En
| MEDLINE
| ID: mdl-2683363
ABSTRACT
A genetic approach was used to facilitate purification of human immunodeficiency virus (HIV) rev protein. A recombinant protein containing a stretch of six histidine residues at the amino terminus was engineered and overexpressed in Escherichia coli. Purification of greater than 95% was achieved in a single step using an immobilized metal ion chromatography with a resin that has selectivity for proteins with neighboring histidine residues. We show that the modified protein is both properly modified and biologically active.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Productos del Gen rev
/
Transactivadores
/
VIH
Límite:
Humans
Idioma:
En
Revista:
Virology
Año:
1989
Tipo del documento:
Article