φXANES: In vivo imaging of metal-protein coordination environments.
Sci Rep
; 6: 20350, 2016 Feb 10.
Article
en En
| MEDLINE
| ID: mdl-26861174
ABSTRACT
We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiation. This method was validated using iron-challenged Caenorhabditis elegans to observe marked alterations in redox environment.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos Organometálicos
/
Procesamiento de Imagen Asistido por Computador
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Caenorhabditis elegans
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Complejos de Coordinación
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Espectroscopía de Absorción de Rayos X
/
Hierro
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Metaloproteínas
Límite:
Animals
Idioma:
En
Revista:
Sci Rep
Año:
2016
Tipo del documento:
Article
País de afiliación:
Australia