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Hydraulic conductivity and aquaporin transcription in roots of trembling aspen (Populus tremuloides) seedlings colonized by Laccaria bicolor.
Xu, Hao; Cooke, Janice E K; Kemppainen, Minna; Pardo, Alejandro G; Zwiazek, Janusz J.
Afiliación
  • Xu H; Department of Renewable Resources, University of Alberta, Edmonton, T6G 2E3, Canada.
  • Cooke JE; Department of Biological Sciences, University of Alberta, Edmonton, T6G 2E9, Canada.
  • Kemppainen M; Laboratorio de Micología Molecular, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bernal, Argentina.
  • Pardo AG; Laboratorio de Micología Molecular, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bernal, Argentina.
  • Zwiazek JJ; Department of Renewable Resources, University of Alberta, Edmonton, T6G 2E3, Canada. jzwiazek@ualberta.ca.
Mycorrhiza ; 26(5): 441-51, 2016 Jul.
Article en En | MEDLINE | ID: mdl-26861480
ABSTRACT
Ectomycorrhizal fungi have been reported to increase root hydraulic conductivity (L pr) by altering apoplastic and plasma membrane intrinsic protein (PIP)-mediated cell-to-cell water transport pathways in associated roots, or to have little effect on root water transport, depending on the interacting species and imposed stresses. In this study, we investigated the water transport properties and PIP transcription in roots of aspen (Populus tremuloides) seedlings colonized by the wild-type strain of Laccaria bicolor and by strains overexpressing a major fungal water-transporting aquaporin JQ585595. Inoculation of aspen seedlings with L. bicolor resulted in about 30 % colonization rate of root tips, which developed dense mantle and the Hartig net that was restricted in the modified root epidermis. Transcript abundance of the aspen aquaporins PIP1;2, PIP2;1, and PIP2;2 decreased in colonized root tips. Root colonization by JQ585595-overexpressing strains had no significant impact on seedling shoot water potentials, gas exchange, or dry mass; however, it led to further decrease in transcript abundance of PIP1;2 and PIP2;3 and the significantly lower L pr than in non-inoculated roots. These results, taken together with our previous study that showed enhanced root water hydraulics of L. bicolor-colonized white spruce (Picea glauca), suggest that the impact of L. bicolor on root hydraulics varies by the ectomycorrhiza-associated tree species.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Raíces de Plantas / Regulación de la Expresión Génica de las Plantas / Acuaporinas / Populus / Plantones / Laccaria Idioma: En Revista: Mycorrhiza Asunto de la revista: BIOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Canadá

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Raíces de Plantas / Regulación de la Expresión Génica de las Plantas / Acuaporinas / Populus / Plantones / Laccaria Idioma: En Revista: Mycorrhiza Asunto de la revista: BIOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Canadá
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