Mutagenesis for improvement of activity and thermostability of amylomaltase from Corynebacterium glutamicum.

Nimpiboon, Pitchanan; Kaulpiboon, Jarunee; Krusong, Kuakarun; Nakamura, Shigeyoshi; Kidokoro, Shun-ichi; Pongsawasdi, Piamsook

Nimpiboon, Pitchanan; Kaulpiboon, Jarunee; Krusong, Kuakarun; Nakamura, Shigeyoshi; Kidokoro, Shun-ichi; Pongsawasdi, Piamsook.

Afiliación

Nimpiboon P; Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Starch and Cyclodextrin Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Kaulpiboon J; Department of Pre-Clinical Science, Biochemistry, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand.
Krusong K; Starch and Cyclodextrin Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Nakamura S; Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan.
Kidokoro S; Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan.
Pongsawasdi P; Starch and Cyclodextrin Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: piamsook.p@chula.ac.th.

Int J Biol Macromol ; 86: 820-8, 2016 May.

Article en En | MEDLINE | ID: mdl-26875536

Asunto(s)

Corynebacterium glutamicum/enzimología; Sistema de la Enzima Desramificadora del Glucógeno/química; Sistema de la Enzima Desramificadora del Glucógeno/metabolismo; Mutagénesis Sitio-Dirigida; Temperatura; Ciclodextrinas/metabolismo; Estabilidad de Enzimas; Regulación Enzimológica de la Expresión Génica; Sistema de la Enzima Desramificadora del Glucógeno/genética; Modelos Moleculares; Mutación; Conformación Proteica; Especificidad por Sustrato

Palabras clave

Amylomaltase; Corynebacterium glutamicum; Large-ring cyclodextrin; Random mutagenesis; Site-directed mutagenesis; Thermostability

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Temperatura / Sistema de la Enzima Desramificadora del Glucógeno / Mutagénesis Sitio-Dirigida / Corynebacterium glutamicum Idioma: En Revista: Int J Biol Macromol Año: 2016 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Países Bajos

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